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Tuesday, July 21, 2020 | History

2 edition of effect of forskolin on the haematopoietic cell-line U937. found in the catalog.

effect of forskolin on the haematopoietic cell-line U937.

Claire M. S. Clyde

effect of forskolin on the haematopoietic cell-line U937.

by Claire M. S. Clyde

  • 69 Want to read
  • 24 Currently reading

Published by The Author] in [S.l .
Written in English


Edition Notes

Thesis (M. Sc. (Biomedical Sciences)) - University of Ulster, 1993.

The Physical Object
Pagination[v], 47p., tables, 4p. of plates :
Number of Pages47
ID Numbers
Open LibraryOL21162561M

  Studies on knockout mice have identified Free Fatty Acid Receptor 2 (FFAR2 or GPR43) as a critical gene in the prevention of obesity, colitis, asthma and arthritis 1,2,3,4,5,6,7,43 is a G. Results In this study, we investigated the effect of fibrate pretreatment on the immune response during M. smegmatis infection in U cells, a human leukemic monocyte lymphoma cell line.

Claire M. S. Clyde has written: 'The effect of forskolin on the haematopoietic cell-line U' Asked in Authors, Poets, and Playwrights What has the author Clyde McNeill written? The Apoptotic and Inhibitory Effects of Phylloquinone in the U Cell Line by Tesha Blair Phylloquinone is a natural analog of vitamin K that has been shown to both inhibit cancer cell growth and induce apoptosis in several cancer cell lines. This study examined these effects in a non-Hodgkin lymphoma cell line, known as U

  Histamine (HA) is approved for the treatment of acute myeloid leukemia (AML). Its antileukemic activity is related to histamine H2-receptor (H2R)-mediated inhibition of reactive oxygen . Melatonin (MEL), a methoxyindole synthesized by the pineal gland, is a powerful antioxidant in tissues as well as within cells, with a fundamental role in ameliorating homeostasis in a number of specific .


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Effect of forskolin on the haematopoietic cell-line U937 by Claire M. S. Clyde Download PDF EPUB FB2

The present study examines the effects of forskolin on U cell differentiation. We recently reported that dibutyryl cAMP (dbcAMP), but not cAMP-elevating agents such as histamine, promotes U cell Cited by: The differentiating effect of dbcAMP on the U cell line, thus, seems to involve activation of protein kinase A.

The natural diterpene, forskolin, binds and stimulates adenylyl cyclase by a direct Cited by: U is a pro-monocytic, human myeloid leukaemia cell line and was isolated from the histiocytic lymphoma of a 37 year old male (Sundstrom and Nilsson ).

This cell line exhibits many Cited by:   FIGURE 1. Effects of forskolin on the sensitivity of U cells to GSKJ4. Panels A, B: U cells were treated or not with 1 and 10 μM GSKJ4 alone or in combination with 10 μM forskolin Cited by: 3. Only in the promyelocytic leukemia cell line HL has spontaneous terminal differentiation been documented (Collins et al.

; Gallagher et al. Recently, however, inducible differentiation in vitro has been demonstrated in three non-lymphoid hematopoietic. Included in the panel was a third hematopoietic cell type, the U cell line originating from a monocytic lymphoma.

As with the K and EU-1 cell lines, 10 5 cells were transduced per. The U cell line is a human haematopoietic cell line established from a generalized histiocytic lymphoma, displaying several properties of immature monocytic cells. Since its establishment, the U cell line has been extensively utilized as a powerful in vitro model for the study of haematopoietic.

FIGURE 1 | Effects of forskolin on the sensitivity of U cells to GSKJ4. Panels A, B: U cells were treated or not with 1 and 10 µ M GSKJ4 alone or in combination with 10 µ M forskolin for.

The NB4 cell line 27 was a gift from Consistent with previous reports, 33 GM-CSF alone showed a minor effect on HL and U The Leukemia–Lymphoma Cell Line Facts Book. This result indicates that the effect of ubenimex on inhibition of U growth is not due to en- hancement of TNF production.

Effect of Bcstatin on hematopoietic cells 75 Table I. Inhibitory effects of. RESULTS. Forskolin Stimulates Macrophage Proliferation, [3 H]Thymidine Uptake, and Phosphorylation of CREB at Ser—Forskolin treatment increased macrophage number in a dose-dependent manner, and this proliferative effect was optimal at ∼10–20 μ m of forskolin (Fig.

1A).Likewise, forskolin increased [3 H]thymidine uptake by macrophages, which was optimal at ∼20 μ m of forskolin. The myelomonocytic cell line THPBlue can be used as a convenient alternative, and both assays can be easily applied for the testing of chemicals but the effects of compounds on the T-cell.

Summary. The effects of ubenimex (Bestatin ®) on proliferation and differentiation in hematopoietic cells were investigated using recombinant erythropoietin (EPO), granulocyte-macrophage colony.

Forskolin extract supplement is made from the root of a plant in the mint family. WebMD informs you about the health benefits, uses, and side effects of this supplement.

Here, we investigate the effects of forskolin on the sensitivity of human leukemia U cells to GSKJ4 through flow cytometry-based assays (cell-cycle progression and cell death), cell number.

Claire M. Clyde has written: 'The effect of forskolin on the haematopoietic cell-line U' Asked in Human Anatomy and Physiology What is the location of blood-forming tissue.

Claire M. Clyde has written: 'The effect of forskolin on the haematopoietic cell-line U' Asked in Herbal Medicine Has anyone tried Pure Natural Forskolin Is it safe and worth the money. VitD3 (1α,dihydroxyvitamin D3; 1,25D) has been recognized to induce differentiation of U cell line.

14 U cells (1×10 6 /ml) containing 5× M concentration of VitD3 (Sigma-Aldrich, USA) were. In M1 mouse myeloid leukemia cells as well as in the human promonocytic U cell line, dibutyryl cAMP (dbcAMP) induces monocyte maturation.

Cyclic AMP can also potentiate granulocytic. Antiproliferative Effect of Liposome Encapsulated PITC-2 on Cancer Cell Line (U, K and HL60) in Vitro Article (PDF Available) June with Reads How we measure 'reads'. Cell line. The human leukemia cell lines U and KG-1 were obtained from the National Cell Bank of Iran (Pasteur Institute, Tehran, Iran).

These cell lines were cultured in complete RPMI medium with 10% and 20% heat inactivated FBS for U .Cells. The mouse melanoma cell line B78H1 and the human histiocytic lymphoma cell line U were purchased from ATCC. Cells were grown at 37 °C in a humidified atmosphere of 5% CO 2 and 95% .PU.1 affects proliferation of the human acute myeloid leukemia U cell line by directly regulating MEIS1 JING ZHOU, 1, 2, * XIAOFENG ZHANG, 3, * YUHUA WANG, 2, 4 and YINGHUI GUAN 5 1 .